Application scenarios of cultivation boards

Cell culture plates are a biological tool. In practical applications, we can see culture plates with different shapes and pore numbers. So, what are the types of cell culture plates and how are they applied?

Cell culture plates can be divided into flat and round bottoms (U-shaped and V-shaped) according to the shape of the bottom, and different shaped culture plates have different purposes. Cultivating cells is usually done using a flat bottom, which facilitates microscopic observation, has a clear bottom area, and the height of the cell culture liquid surface is relatively consistent. Therefore, when conducting experiments such as MTT, whether it is adherent or suspended cells, a flat bottom plate is generally used. The absorbance value must be measured using a flat bottomed culture plate. On the material, the label "Tissue Culture (TC) Processed" is used for nourishing cells.

U-shaped or V-shaped cell culture plates are generally only used in certain special requirements. In immunology, when two different types of lymphocytes are mixed and cultured, they need to be in contact with each other to stimulate each other. In this case, U-shaped plates are generally used because cells will aggregate within a small range due to the effect of gravity. The round bottom culture plate can also be used for isotope doping experiments, requiring the collection of cell cultures using a cell collection instrument, such as "mixed lymphocyte culture". V-shaped plates are commonly used for cell killing and immunological blood agglutination experiments. Cell killing experiments can also be replaced by U-shaped plates (after adding cells, centrifuge at low speed).

With the rapid advancement of biological research technology, the types of cell culture plates are also changing to better meet the needs of different cell cultures.