The working principle of a pipette is mainly based on piston motion and the generation of vacuum or pressure. Pipettes can be divided into two categories: gas piston systems and external piston systems. Here is a detailed introduction:
1. Gas piston system. This type of system contains air inside, and when the control button is pressed, the piston will push some of the air out of the pipette, creating a vacuum in the suction head to suck in the liquid. After releasing the button, the vacuum section is filled with liquid, which is sucked into the suction head and then discharged by pushing the piston downwards. External piston system. The piston in this system comes into direct contact with the target liquid. When the piston is pulled up, atmospheric pressure squeezes the liquid into the suction head.
In addition, the capacity of the pipette can be set by adjusting the volume control knob of the pipette. The accuracy of a pipette also depends on its design, such as the elasticity of the spring, which determines the suction and discharge capabilities of the pipette.